Studies on .BETA.-galactosidase. I. Purification and properties of .BETA.-galactosidase I and II from Sclerotium tuliparum.
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The purification and properties of beta-galactosidase from bovine testes.
A /3-galactosidase was purified 600-fold from bovine testes by ammonium sulfate precipitation, acetone fractionation, and at5nity chromatography on agarose substituted with terminal thio-fl-galactopyranosyl residues. The preparation was devoid of protease, a-fucosidase, ar-mannosidase, pglucosidase, P-glucuronidase, and hyaluronidase activities. A wide variety of compounds containing terminal n...
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A gene encoding a third alpha-galactosidase (AglB) from Aspergillus niger has been cloned and sequenced. The gene consists of an open reading frame of 1,750 bp containing six introns. The gene encodes a protein of 443 amino acids which contains a eukaryotic signal sequence of 16 amino acids and seven putative N-glycosylation sites. The mature protein has a calculated molecular mass of 48,835 Da...
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Adult rat brain beta-galactosidase was partially purified with the use of lactosylceramide, galactosyl-N-acetylgalactosaminyl-galactosyl-glucosylceramide, galactosyl-N-acetylgalactosaminyl-(N-acetylneuraminyl)galactosyl-glucosylceramide, and 4-methylumbelliferyl theta-galactoside as substrates. Approximately 50-fold purification was achieved by solubilization, ammonium sulfate fractionation, Se...
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ژورنال
عنوان ژورنال: Chemical and Pharmaceutical Bulletin
سال: 1976
ISSN: 0009-2363,1347-5223
DOI: 10.1248/cpb.24.788